JN-QC-SPOT TB

Mycobacterium tuberculosis (Mtb) diagnostic test kit
INTRODUCTION
Mycobacterium tuberculosis is an organism, which is indicated as an etiological agent of Tuberculosis

The rapid test is a qualitative test for TB infection. It offers detection of antibodies to the TB bacterium in fresh whole blood and serum specimen.

Rapid serological test principle

The test called Line Immunobinding Serological Assay (LISA) was made from careful selection of multiple antigens from TB bacterium polypeptides, which are secreted by TB during active infection. LISA TB assay is a rapid immunochromatographic test with a unique cocktail recombinant antigen that may be capable of differentiating antibodies present during active Tuberculosis infection versus dormant. Studies are being conducted to confirm these properties It can be used with serum or whole blood. As the test samples flow laterally through the nitrocellulose membrane, when a sample is added to the sample well it diffuses past and binds to specific antigen line if human immunoglobulin G antibodies against TB are present. Anti-human IgG antibodies coupled to colloidal gold particles binds to the human IgG antibodies produce a pink/purple band. In the absence of Tuberculosis antibodies there is no line in the positive reaction zone. The liquid continues to migrate along the membrane and produces a pink/purple band in the CONTROL zone demonstrating that the reagents are functioning properly. This assay is designed as the T line, a burgundy color band in the test region of membrane, will be developed rapidly when antibodies to TB are present in the specimen. If antibodies to TB are not present, no T line will be developed in the test region. The C line, a burgundy color (see picture on the right)

BIBLIOGRAPHY

* 1. World Health Organization, Laboratory biosafety Manual, Geneva. World Health Organization, 1983.

*2. Reddy, J.R., Kwang, J., Khan, N, K.F. Lechtenberg and Reddy B.P. Immunochromatograhic rapid detection of Mycobacterium tuberculosis antibodies from whole blood and serum. http://www.ncbi.nlm.nih.gov/pubmed/11831744?dopt=Abstract/ PubMed 11831744]

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