Flippase recognition target

In genetics, the Flippase recognition target (FRT) is 34 bp - long site sequence. It is used in FLP-FRT recombination technology for manipulating an organism's DNA under controlled conditions in vivo.

The sequence is: 5'-GAAGTTCCTATTCtctagaaaGTATAGGAACTTC-3'.
Flippase (FLP)) binds to the 13-bp 5'-GAAGTTCCTATTC-3' and to the reverse complement of 5'-GTATAGGAACTTC-3' (5'-GAAGTTCCTATAC-3').
The FRT site is cleaved just before 5'-tctagaaa-3', the 8bp asymmetric core region, on the top strand and behind this sequence on the bottom strand.
Several variant FRT sites exist. Recombination can occur between two identical FRT sites but generally not between non-identical FRT sites
Many available constructs include the sequence
5'-GAAGTTCCTATTCC-3'
immediately upstream the FRT site
(resulting in 5'-GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC-3')
but this sequence is dispensable for recombination.

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