</noinclude> Artificial microRNA (amiRNA) ia a type of miRNA which is derived by replacing native miRNA duplexes from a natural miRNA precursor. Key features of an amiRNA * An amiRNA should have secondary structural similarity that of its precursor. * It can be processed by host machinery as normal miRNA. * After processing it will produce amiRNA:amiRNA* duplex instead of natural miRNA:miRNA* duplex. * It should have the ability to confer PTGS to a specific target. Types of amiRNA * monocistronic amiRNA- amiRNA produces only one amiRNA:amiRNA* duplex * Dicistronic amiRNA- produces two amiRNA:amiRNA duplex of same type or different type * Polycistronic amiRNA- can produce more than two types of amiRNA:amiRNA* duplex Use of amiRNA *amiRNA is a new strategy having huge potential. As a PTGS, it is tested to confer viral resistance in plants. Nonetheless, more research must be done to improve a better strategy which can confer resistance to fungal and bacterial diseases.
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