T-loop deletion factor

T-loop deletion factor (TDF) is a active factor in every human telomeres speeding up the aging and the cancer cascades. As telomere ends of each chromosome shorten on each of the two arms on each side of each chromatid, appears that the RNA primer factor leave gaps 70-200bp, which do not actually play any role in the DNA end-replication problem (Hayflick limit), as the deletions are actually done via this novel t-loop deletion factor, leaving 50-300bp 3’ overhangs on both sides of each chromatid, after each DNA replication, as after each DNA replication there are t-loops forming (Stoyanov, 2009). The t-loop deletion factor explains also the different ratios of telomere blunt ends and how telomere blunt ends result in overhangs on both sides of each chromatid. Thus it is the t-loop deletion factor mechanism, which is responsible for the DNA end replication problem (Hayflick limit). Thus anti aging strategies need to focus on the 5' t-loop deletion factor 50-300bp telomere shortening, instead of the 3’ RNA primer factor (Stoyanov, 2009).
Comparing the current known telomere deletion factors show us why the t-loop deletion factor is so important:
- 50-300bp deletions via the t-loop deletion factor (Stoyanov, 2009) has also effect on every cell division.
- 70-200bp gaps via the RNA primer factor, resulting always in 50% blunt ends (in vitro) (Harley et al, 1990) has no effect on telomere deletions.
- Up to 500bp deletions via the T-loop sized deletions (Wang et al, 2004) has no effect on every cell division, but only in abnormal cases, e.g. cancer.
- About 2000bp deletions on average, via the telomere rapid deletions (TRD) can shorten single telomere by several Kb in a single generation (A. thaliana) (Watson & Shippen, 2007) has no effect on every cell division, but only in abnormal cases, e.g. cancer.

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