AsdA (antisense RNA of dnaA) is a cis-encoded antisense RNA of dnaA described in Salmonella enterica serovar Typhi. It was discovered by deep sequencing and its transcription was confirmed by Northern blot and RACE analysis. AsdA, estimated to be about 540 nucleotides long, is expressed mostly in the stationary phase of the bacterial growth and under iron limitation and osmotic stress. Overexpression of AsdA increases dnaA mRNA level suggesting that the antisense RNA increases the stability of dnaA mRNA enhancing its translation.
The AsdA sRNA should not be confused with protein-coding asdA genes which encode aminotransferases and other enzymes.
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